SUMOproteinpurification

由 F Guerrero 著作 · 2015 · 被引用 91 次 — Fusion tags are versatile tools to facilitate efficient protein purification and to improve soluble overexpression of proteins. Various purification and fusion ...

由 H Wang 著作 · 2010 · 被引用 98 次 — As a novel molecular chaperon domain, SUMO increases the yield of recombinant protein and makes it easier to dissolve in the soluble fraction ...

由 CD Lee 著作 · 2008 · 被引用 152 次 — One can carry out both fusion protein purification and SUMO protease cleavage using one Ni2+-resin column. The eluant contains only the native target protein.

2015年5月5日 — Analyze purified protein by SDS PAGE (4-12 microliters/lane depending on expression level). Sumo protease cleavage and clean up. Change protein ...

由 XF Zhou 著作 · 2020 · 被引用 7 次 — Here, we describe a simple and rapid protein purification strategy in which the SUMO tag and Ulp1 protease are surface-displayed separately on ...

Sumo tag is most frequentely used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein.

由 TR Butt 著作 · 2005 · 被引用 632 次 — This technology has been used to efficiently express and purify a variety of proteins, including membrane proteins [78]. Another innovative application of the ...

The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry and this kit allows a rapid and efficient affinity purification of active SUMO ...

In order to purify such proteins it may be necessary to fuse the protein of interest with a solubility tag such as SUMO or MBP (maltose-binding protein) to ...

The SUMOPro system is designed for expression and purification of proteins from E. coli. ... SUMOstar fusion proteins can be cleaved only with SUMOstar protease.